Stable Microwell Immobilized Lectins

                                (A cost effective, non-destructive, efficient and rapid method

                                 for micro-screening, isolating or assaying many specimens)

Introduction: Sugar binding Lectins have long been used to isolate proteins, and other sugar containing molecules. They are often used to identify sugar moiety's on proteins.  Lectins by definition are proteins having at least two sugar binding sites.  Lectin specificity is defined by the monosaccharides or oligosaccharides that inhibit the agglutination or precipitation which the lectin causes.  The combination of their sugar binding specificity and reversible elution makes them valuable tools for both detection of various proteins and micro-elution.

ALerCHEK offers over 55 commercially available lectins covalently immobilized onto microwell strip plates.  100ul of specimen, standards or unknowns are added to each respective well, incubated, decanted and washed.  The corresponding bound protein, polysaccharide, etc. may be either detected with a corresponding antibody or specifically eluted with the corresponding sugar for further analysis, e.g. SDS-Page, etc. .